Modified Diphasic Liver Infusion Agar: Selective Medium for the Axenic Cultivation of Entamoeba histolytica

Main Article Content

Zuhair W. Dardona
Adnan I. Al-Hindi
Samia Boussaa

Abstract

A modified diphasic liver infusion agar medium was prepared manually in the laboratory in order to be used for culturing Entamoeba histolytica in vitro. This medium is a good alternative for the commercial one and has the main ingredients for the parasite's growth.  E. histolytica was successfully cultured in the present study and PCR technique was performed to confirm Entamoeba species. The growth of E. histolytica trophozoites had started in the third day post-cultivation and reached its highest rate in the seventh. Then the growth started to decrease until the trophozoites completely disappeared after 12-14 days post-cultivation. Cryopreservation using glycerol 10% (V/V) was used in order to preserve trophozoites for later cultivation.

Keywords:
E. histolytica, diphasic, liver infusion agar, PCR, trophozoites, cultivation, cryopreservation

Article Details

How to Cite
W. Dardona, Z., I. Al-Hindi, A., & Boussaa, S. (2019). Modified Diphasic Liver Infusion Agar: Selective Medium for the Axenic Cultivation of Entamoeba histolytica. South Asian Journal of Parasitology, 3(1), 1-6. Retrieved from http://journalsajp.com/index.php/SAJP/article/view/30087
Section
Original Research Article

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