Ovicidal Effect of Tannins-containing Extract of Sesbania grandiflora Leaves against Goat’s Parasitic Nematode Egg

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M. Azrul-Lokman
W. Arirop
K. Poungpong
S. Jittapalapong
S. Prasanpanich


Aims: In this study, sesbania (Sesbania grandiflora) leaves were used as an experimental material. Tannins was measured using Folin-Ciocalteu method. The ovicidal effect of this plant leaves was determined against goat’s parasitic nematode egg using egg hatch assay.

Study Design: Completely Randomized Design (CRD).

Place and Duration of Study: Nematode eggs collection and in vitro trial were conducted at Animal Physiology Laboratory, Faculty of Agriculture of Kasetsart University in Bangkok, Thailand between June 2016 and December 2016.

Methodology: Two type of extracts; aqueous and ethanolic extracts were prepared prior to the in vitro trial. Six increasing 2-fold concentration of both extracts; from 0.075 to 2.4 mg/ml were used against mixed sample of nematode’s egg from naturally infected goats. Gridded petri dishes containing a mixture of egg suspension with tested solution; both extracts and control were incubated for 27°C for 24hrs and 48hrs, respectively. At the end of each incubation period, hatched and unhatched eggs were counted and egg hatching inhibition rate was determined.

Results: Total tannins in this plant was measured at 1.08% of 95% dry matter (DM). Both extracts showed significant inhibition effect for both incubation periods (P<0.05). Highest concentration used for aqueous and ethanolic extracts showed more than 80% and 60% of hatching inhibition after 48hrs, respectively. Significantly higher inhibition rate was observed for aqueous extract compared to ethanolic extract (P<0.05). For aqueous extract, the maximum inhibition rate was observed at 85.33% for the highest concentration after 48hrs which is almost similar to anthelmintic efficacy of oxfendazole used as positive control (96.67%).

Conclusion: This study showed that S. grandiflora leaves can be used as natural anthelmintic for goats since their ovicidal effect against nematode’s egg was scientifically proven.

Sesbania grandiflora, goat’s parasitic nematode egg, egg hatch assay, egg hatching, inhibition rate, ovicidal effect.

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Azrul-Lokman, M., Arirop, W., Poungpong, K., Jittapalapong, S., & Prasanpanich, S. (2019). Ovicidal Effect of Tannins-containing Extract of Sesbania grandiflora Leaves against Goat’s Parasitic Nematode Egg. South Asian Journal of Parasitology, 3(1), 1-8. Retrieved from http://journalsajp.com/index.php/SAJP/article/view/30089
Original Research Article


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