Ovicidal Effect of Tannins-containing Extract of Sesbania grandiflora Leaves against Goat’s Parasitic Nematode Egg

Main Article Content

M. Azrul-Lokman
W. Arirop
K. Poungpong
S. Jittapalapong
S. Prasanpanich

Abstract

Aims: In this study, sesbania (Sesbania grandiflora) leaves were used as an experimental material. Tannins was measured using Folin-Ciocalteu method. The ovicidal effect of this plant leaves was determined against goat’s parasitic nematode egg using egg hatch assay.

Study Design: Completely Randomized Design (CRD).

Place and Duration of Study: Nematode eggs collection and in vitro trial were conducted at Animal Physiology Laboratory, Faculty of Agriculture of Kasetsart University in Bangkok, Thailand between June 2016 and December 2016.

Methodology: Two type of extracts; aqueous and ethanolic extracts were prepared prior to the in vitro trial. Six increasing 2-fold concentration of both extracts; from 0.075 to 2.4 mg/ml were used against mixed sample of nematode’s egg from naturally infected goats. Gridded petri dishes containing a mixture of egg suspension with tested solution; both extracts and control were incubated for 27°C for 24hrs and 48hrs, respectively. At the end of each incubation period, hatched and unhatched eggs were counted and egg hatching inhibition rate was determined.

Results: Total tannins in this plant was measured at 1.08% of 95% dry matter (DM). Both extracts showed significant inhibition effect for both incubation periods (P<0.05). Highest concentration used for aqueous and ethanolic extracts showed more than 80% and 60% of hatching inhibition after 48hrs, respectively. Significantly higher inhibition rate was observed for aqueous extract compared to ethanolic extract (P<0.05). For aqueous extract, the maximum inhibition rate was observed at 85.33% for the highest concentration after 48hrs which is almost similar to anthelmintic efficacy of oxfendazole used as positive control (96.67%).

Conclusion: This study showed that S. grandiflora leaves can be used as natural anthelmintic for goats since their ovicidal effect against nematode’s egg was scientifically proven.

Keywords:
Sesbania grandiflora, goat’s parasitic nematode egg, egg hatch assay, egg hatching, inhibition rate, ovicidal effect.

Article Details

How to Cite
Azrul-Lokman, M., Arirop, W., Poungpong, K., Jittapalapong, S., & Prasanpanich, S. (2019). Ovicidal Effect of Tannins-containing Extract of Sesbania grandiflora Leaves against Goat’s Parasitic Nematode Egg. South Asian Journal of Parasitology, 3(1), 1-8. Retrieved from http://journalsajp.com/index.php/SAJP/article/view/30089
Section
Original Research Article

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