Experimental Infection with Foodborne Parasite (Trichinella spiralis) Induced Damage and Apoptosis in Rat Skeletal Muscle Fibers

Lamiaa Bakr

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.

Areej Jameel M. Alghabban

Biology Department, Faculty of Science, University of Tabuk, KSA.

Afaf El Atrash

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.

Aya A. Elbatawy

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.

Ehab Tousson *

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.

*Author to whom correspondence should be addressed.


Aims: Apoptosis, a type of host-cell death, frequently causes tissue damage in the deaths caused by parasite infections. Current study aimed to study the histopathological and Immunohistochemical changes in rats skeletal muscles after experimental trichinellosis infection.

Methodology: A total of 20 male rats (Sprague Dawley) were divided into 2 groups (Negative control and positive control or Infected). At the end of the experiment; skeletal muscles fron control and infected rats were fixed in 10% formalin and examined for histopathological changes using haematoxylin and eosin stains and Immunohistochemical changes against a poptotic P53 proteins and anti-apoptotic Bcl2 immunoreactivities.

Results: Current results revealed that; diffuse degenerative changes all over the muscle fibers with massive numbers of T. spiralis encysted larvae that surrounded by a collagen capsule and mild inflammatory cellular infiltration in addition to elevation in apoptotic P53 proteins and reduction in anti-apoptotic Bcl2 in addition to muscle muscles injury.

Conclusion: It can conclude that; experimental infection with Trichinella spiralis can induce damage and apoptosis in rat skeletal muscle fibers.

Keywords: Trichinella spiralis, rats, apoptosis, skeletal muscles

How to Cite

Bakr , L., Alghabban , A. J. M., Atrash , A. E., Elbatawy , A. A., & Tousson , E. (2023). Experimental Infection with Foodborne Parasite (Trichinella spiralis) Induced Damage and Apoptosis in Rat Skeletal Muscle Fibers. South Asian Journal of Parasitology, 6(2), 44–50. Retrieved from https://journalsajp.com/index.php/SAJP/article/view/151


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Dorny P, Praet N, Deckers N, Gabriel S. Emerging food-borne parasites. Veterinary Parasitology. 2009;163:196–206.

Zhang N, Li W, Fu B. Vaccines against Trichinella spiralis: Progress, challenges and future prospects. Transboundary and Emerging Diseases. 2018;65(6):1447-1458.

Murrell KD, Pozio E. Worldwide occurrence and impact of human trichinellosis, 1986–2009. Emerging Infectious Diseases. 2011;17(12): 2194.

Neghina R, Moldovan R, Marincu I, Calma CL, Neghina AM. The roots of evil: The amazing history of trichinellosis and Trichinella parasites. Parasitology Research. 2012;110:503-508.

Saad AE, Ghanem HB. Trichinella spiralis as a potential therapeutic agent: From a risky disease to a friend. Journal of the Egyptian Society of Parasitology. 2020; 50(1):119-126.

Meagher S, Dudek SN. Effects of Trichinella spiralis on survival, total mass, and organ mass of oldfield mice (Peromyscus polionotus). Journal of Parasitology. 2002;88(5):833-838.

Watt G, Saisorn S, Jongsakul K, Sakolvaree Y, Chaicumpa W. Blinded, placebo-controlled trial of antiparasitic drugs for trichinosis myositis. The Journal of Infectious Diseases. 2000;182(1):371-374.

Gottstein B, Pozio E, Nöckler K. Epidemiology, diagnosis, treatment, and control of trichinellosis. Clin Microbiol Rev. 2009;22(1):127–145.

Tousson E, Beltagy DM, Gazia MA, Al-Behbehani B. Expressions of P53 and CD68 in mouse liver with Schistosoma mansoni infection and the protective role of silymarin. Toxicology and industrial health. 2013;29(8):761-770.

El-Aarag B, Attia A, Zahran M, Younes A, Tousson E. New phthalimide analog ameliorates CCl4 induced hepatic injury in mice via reducing ROS formation, inflammation, and apoptosis. Saudi Journal of Biological Sciences. 2021;28(11):6384-95.

Karmańska K, Houszka M, Piekarska J. The phenomenon of apoptosis in the course of experimental trichinellosis in mice. Wiad Parazytol. 2000;46:111–115.

Boonmars T, Wu Z, Nagano I, Takahashi Y. Trichinella pseudo spiralis infection is characterized by more continuous and diffuse myopathy than T. spiralis infection. Parasitol Res. 2005;97:13–20.

Dunn IJ, Wright KA. Cell injury caused by Trichinella spiralis in the mucosal epithelium of B10A mice. The Journal of Parasitology. 1985;757-766.

Tousson E. Histopathological alterations after a growth promoter boldenone injection in rabbits. Toxicology and Industrial Health. 2016;32(2):299-305.

Tousson E, Hafez E, Zaki S, Gad A. P53, Bcl-2 and CD68 expression in response to amethopterin-induced lung injury and ameliorating role of l-carnitine. Biomedicine & Pharmacotherapy. 2014;68(5): 631-9.

Tousson E, Hafez E, Zaki S, Gad A. The cardio protective effects of L carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin. Environmental Science and Pollution Research. 2016;23(20):20600-8.

Mido S, Fath EM, Farid AS, Nonaka N, Oku Y, Horii Y. Trichinella spiralis: Infection changes serum paraoxonase-1 levels, lipid profile, and oxidative status in rats. Experimental Parasitology. 2012; 131(2):190-194.

Basyoni MM, El-Sabah AA. Therapeutic potential of myrrh and ivermectin against experimental Trichinella spiralis infection in mice. Korean J. Parasitol. 2013;51(3):297- 304.

Etewa SE, Fathy GM, Abdel-Rahman SA, El-Khalik DA, Sarhan MH, Badawey MS. The impact of anthelminthic therapeutics on serological and tissues apoptotic changes induced by experimental trichinosis. Journal of Parasitic Diseases. 2018;42(2):232–242.

Ibrahim S, Sarhan MH, Farag TI, Mohamed AH. Apoptic and vascular changes in Trichinella spiralis infected mice after parenteral artmether treatment. Journal of the Egyptian Society of Parasitology. 2019;49(1):17-27.