Prevalence of Root-knot Nematode (Meloidogyne Species) on Waterleaf (Talinum triangulare) in Three Locations in University of Calabar, Nigeria

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Daniel Offiong Etim
Eleng, Ituna Emini
Bassey, Rosemary Anietie
Igwe, Chidimma Blessing


Aim: A comprehensive survey of root-knot nematodes was conducted in three locations (Unical farm, Biological Science and Botanical Garden) between June, 2018 to August, 2018 to determine the intensity of Meloidogyne incognita and Meloidogyne hapla on the three locations of University of Calabar, Nigeria.

Methodology: A randomized sampling pattern was used during sampling, were a total of 60 samples (30 infected roots and 30 infected soil) samples were collected from the three major locations. In each location, 10 sampling points were selected randomly. Data were collected on Number of galls per root system, Gall index (G.I), Nematode density in soil and Number of nematode per plant. Data was analyzed using descriptive statistics of chi-square.

Results: The prevalence of Root-knot nematodes was recorded with varying degree in all locations. The highest prevalence of Root-knot nematodes (M. incognita) 62.25% with (G.I = 4.00) was recorded from Botanical garden followed by 39.12% with (G.I = 4.00) in Biological Science block compared to Unical farm which recorded minimum prevalence of 15.41% with (G.I = 3.00). The result of the survey obtained showed that (M. incognita and M. hapla are prevalence in all the three locations. However, Unical farm had the least Root-knot infection of (M. hapla) compare to (M. incognita).

Conclusion: The results of this study showed that the composition of nematode communities (plant-parasitic and free-living) may be used as bio indicators of soil health or condition. This further suggests that magnitude of nematode problem needs serious consideration to tackle by the use of useful nematode management strategies. 

Survey, Meloidogyne species, waterleaf, soil health, University of Calabar

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Etim, D. O., Emini, E. I., Anietie, B. R., & Blessing, I. C. (2020). Prevalence of Root-knot Nematode (Meloidogyne Species) on Waterleaf (Talinum triangulare) in Three Locations in University of Calabar, Nigeria. South Asian Journal of Parasitology, 4(3), 18-26. Retrieved from
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Jones JT, Haegemen A, Danchin EGJ, Gaur HS, Helder J, Jones MGK, Kikuchi T, Palomares-Rius JE, Wesemael WML, Perry RN. Top 10 plant-parasitic nematodes in molecular plant pathology. Mol Plant Pathol. 2013;14:946-961.

Gill HK, Mcsorley R. Cover crops for managing root-knot nematodes. university of florida, IFAS Extension, ENY. 2011; 3:1–6.

Karajeh M. Interaction of root-knot nematode (Meloidogyne javanica) and tomato as affected by hydrogen peroxide. J Plant Prot Res. 2008;48(2):2.

Sasser JN. Root-knot nematodes: A global menaced to crop production. Plant Disease. 1980;64(1):36-41.

Abad P, Favery B, Rosso MN, Castagnone-Sereno P. Root-knot nematode parasitism and host response: Molecular basis of a sophisticated interaction. Mol Plant Pathology. 2003; 4:217–224.

Olsen MW. Root-knot Nematode. University of Arizona, Arizona Cooperative Extension, AZ. 2000;1187:1–3.

Udoh EJ, Etim NA. Measurement of farm level efficiency of waterleaf (Talinum triangulare) production among city farmers in Akwa Ibom State, Nigeria. Journal of Sustainable Development in Agriculture and Environment. 2008;3(2):47-54.

Nyananyo BL, Olowokudego JD. Taxonomic studies in the genus Talinum in Nigeria. Wildnowia. 1986;15:455-463.

Akachuku CO, Fawusi MAO. Growth characteristics, yield and nutritional value of waterleaf, Talinum triangulare (Jacq) wild in a semi-wild environment. Discovery and Innovation. 1995;7:163-172.

Sikora RA, Fernandez E. Nematode parasites of vegetables. Pp 319-392. In Luc M, Sikora RA, Bridge J, (eds). Plant-Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd Edition. CAB International; 2005.

Khan MT, Khan MW. Effect of ammonia and root-knot nematode on tomato. Agriculture Ecosystem and Environment. 1994;53:71-81.

Shurleff MC, Averre, CW. Diagnosing plant disease caused by plant parasitic nematodes. The American Phytopathol Society. 2000:187.

Caveness FE. End of tour progress report on Nematology. Ministry of Agriculture and Natural Resources; 1967.

Fawole B, Egunjobi OA, Adesiyan SO, Babatola OA, Idowu AA. The biology and control of nematode pests of food crops in Africa. Heineman Educational Books (Nig) ltd. 1992:67-98.

Osei K, Addico R, Nafeo A, Edu-Kwarteng A, Agyemang A, Danso Y, Sackey-asante j. effect of some organic waste extracts on hatching of meloidogyne incognita eggs. Afr. Jour. of Agric. Res. 2011;6(10):2255-2259.

Stonton J. Tomato root knot nematodes: Biology and control. Department of Primary Industries and Fisheries. G. Stirling (edn.), Biological Crop Protection; 2001.

Widmer TL, Ludwig JW, Abawi GS. The northern root-knot nematodes on carrot, lettuce and onions in New York. New York Food and Science Bulletin; 2005.

Sikora RA, Greco N. Nematode parasites of food legumes In: Plant parasitic nematodes in sub-tropical agriculture. CAB International Institute of Parasitology Walling Food, United Kingdo. 1990; 8(4):523-629.

Mussarrat J, Haseeb A. Agrichemicals as antagonist of lectin-mediated rhizobium- legume symbiosis: Paradigms and Prospects. Curr. Sci. 2000;78:793-797.

Adesiyan SO. Nematode pests of tropical crops. Ibadan, Oyo State: Heinemann Educational Books, Nigeria Limited; 1990.

Akpheokhai IL, Cladius-Cole AO, Fawole B. Evaluation of some plant extracts for the management of meloidogyne incognita on Soybean (Glycin max). World J. Agric. Sci. 2012;8(4):429-435.

Adesiyan SO. The efficacy of carbofuran (furadan) in controlling Meloidogyne incognita in water leaf (Talinum triangulare). Nigerian Journal of Agricultural Sciences. 1992;1(1),22-27.

Alalade OA, Matanmi BM, Olaoye IJ, Adegoke BJ, Olaitan TR. Assessment of pests control methods and its perceived effect on agricultural production among farmers in Kwara State, Nigeria. Journal of Tropical Agriculture, Food, Environment and Extension. 2017;16(1):42-47.

Taylor AL, Sasser JN. Biology, identification and control of root-knot nematodes (Meloidogyne species). North Carolina State University, USAID, NC Graphics, Raleigh; 1978.

Iwena OA. Essential geography, Ibadan, Oyo, Tonal Publisher Limited; 2008.

Coyne DL, Nicol JM, Claudius-Cole B. Practical plant nematology: A field and laboratory guide. Benin, Cotonou, International Institute of Tropical Agriculture. (IITA) Printing Press; 2014.

Byrd DW, Kirkpatrick T, Baker KR. An improved technique for clearing and staining plant tissue for detection of nematodes, Journal of Nematology. 1983; 15:142-143.

Taylor DP, Netscher C. An improved technique for preparing perineal patterns of Meloidogyne spp. Nematologica. 1974; 20:268-269.

Khan HU, Mukhtar T, Ahmad R. Geographical distribution of root-knot nematodes (Meloidogyne spp.) in the Punjab Province of Pakistan. Pakistan Journal of Nematology. 2005;23(1):133-140.

Shahid M, Rehman AU. Khan AU, Mahmood A. Geographical distribution and infestation of plant parasitic nematodes on vegetables and fruits in the Punjab province of Pakistan. Pakistan Journal of Nematology. 2007;25(1):59-67.

FAO. Food and Agricultural Organisation of the United nation on-line and Multilingual Database; 2006.

Available: Retrieved 25th August, 2017.

Sasser JN, Carter CC. The international Meloidogyne project- its goals and accomplishment. Annual Review of Phytopathology. 1985;21:271-288.

Singh RS, Sikora RA, Beniwal SP. Observation on the effect of sawdust on the incidence of root- knot nematode and yield of okra and tomatoes in nematode infested soil. Plant Disease Reporter. 1967;51:861-863.

Haroon TH, Zylstru SN. Rapid identification of genetic relationship of the M. incognita population by polymerase chain reaction RAP makers Egyptian. Journal of Agro-nematology. 2003;32(4):18-20.

Netsher C. Observation and preliminary studies on the occurrence of resistance breaking biotypes biotypes of Meloidogyne specie on tomato. Cah. ORSTOM. Serial Biology. 1985;11:173-178.

Aung T, Prot JC. Effects of crop rotations on Praryletdiils zeae and yield of rice cultivar UPL Ri-5. Revue de Nématologie. 1990;13:445-448.

Chindo PS, khan FA. Effects of soil organic amendment with poultry manure on the damage caused by the Root-knot nematode Meloidogyne incognita on tomato. International Network Newsletter, 1986;3(4):30-33

Aslam SN, Newman MA, Erbs G, Morrissey KL, Chinchilla D, Boller T, Jensen T, De Castro C, Ierano T, Molinaro A, Jackson RW, Knight MR, Cooper RM. Bacterial polysaccharides suppress induced innate immunity by calcium chelation. Curr. Biol. 2008;18:1078–1083.

Nwanguma EI, Awoderu JB. The relevance of poultry and pig droppings as nematode suppressants on okra and tomato in Ibadan, South Western Nigeria. Nigerian Journal of Horticultural Sciences. 2002;6: 67-69.

Floret C, Serpantié G. La jachère en Afrique de l’Ouest. ORSTOM, Colloques et Séminaires, Paris; 1993.