In vivo Alpha Amylase and Alpha Glucosidase Inhibitory Activities of Hippocratea africana Root Extract and Fractions
South Asian Journal of Parasitology,
Aim: To study the in vivo alpha amylase and alpha glucosidase inhibitory activities of Hippocratea africana root extract and fractions.
Methods: The root extract (200, 400, 600 mg/kg) and fractions (n-hexane, dichloromethane, ethyl acetate and methanol, 400 mg/kg) of H. africana were evaluated in rats for inhibitory effect on alpha amylase and alpha glucosidase enzymes using starch, sucrose and maltose as substrates. The substance used for reference was Acarbose.
Results: The effect of extract resulted in significant (P.˂.05) reduction in blood glucose levels of treated rats though non dose-dependently with the various substrates used. The ethyl acetate fraction exerted the highest inhibitory effect when starch was used as a substrate, while ethyl acetate followed by methanol were the most active fractions when maltose and sucrose were used as substrates.
Conclusion: The results suggest that the root extract and fractions of H. africana have the potentials to inhibit alpha amylase and alpha glucosidase in rats and thus reduce blood glucose levels.
- alpha amylase
- alpha glucosidase
- Hippocratea africana
How to Cite
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