South Asian Journal of Parasitology 2020-11-20T06:31:29+00:00 South Asian Journal of Parasitology Open Journal Systems <p style="text-align: justify;"><strong>South Asian Journal of Parasitology</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/SAJP/general-guideline-for-authors">Click here for Types of paper</a>)&nbsp;all areas of basic and applied parasitology.</p> Prevalence and Risk Factors of Intestinal Helminth Infections among City Dwellers of Dhaka, Bangladesh 2020-10-21T11:56:53+00:00 Mandira Mukutmoni Sumaiya Islam Hamida Khanum <p><strong>Aims:</strong> The aim of the study was to determine intestinal helminth prevalence and risk factors among urban dwelling people of Dhaka, Bangladesh.</p> <p><strong>Study Design:</strong> Cross sectional study.</p> <p><strong>Place and Duration of the Study:</strong> A total of 78 stool samples were collected from the residents of Shantinagar area of Dhaka, Bangladesh from January 2019 to August 2019.</p> <p><strong>Methodology:</strong> The respondents were requested to provide their stool samples in supplied container and to fill up a questionnaire regarding their demographic background and household practices. Formol Ether Concentration technique was applied to process the stool samples.</p> <p><strong>Results:</strong> Of the 78 respondents, 42 (53.85%) were infected with at least one helminth. <em>Ascaris lumbricoides </em>(23.08%) showed the highest prevalence following <em>Taenia</em> sp. (11.54%). Education, pattern of consuming antihelminthic drugs, trends of footwear use, eating raw vegetables and undercooked meat were significant risk factors. Respondents who had idea about transmission mode of parasites showed high prevalence of intestinal helminths. Enteric troubles were noticed among the helminth infected respondents.</p> <p><strong>Conclusion:</strong> Increase of hygiene practices among the urban dwellers is essential.</p> 2020-10-21T00:00:00+00:00 ##submission.copyrightStatement## Malaria Parasitaemia and Intensity among Voluntary Blood Donors in Port Harcourt Metropolis, Rivers State, Nigeria 2020-10-21T12:04:17+00:00 Afam Chibuike Okafor Chinyelu Angela Ekwunife Pauline Ukamaka Umeanaeto Kindness Chidi Irikannu <p><strong>Aim:</strong> To investigate malaria parasitaemia and intensity of infection in voluntary blood donors in Port Harcourt Metropolis, Rivers State, Nigeria.</p> <p><strong>Study Design:</strong> The study was a cross sectional survey, cutting across communities, age, sex, occupational and educational groups. A total of 200 voluntary blood donors within the ages of 15 and 65 years, participated in the study.</p> <p><strong>Duration of Study:</strong> The study was conducted between March 2017 and June 2018 at the Department of Pathology, River State University Teaching Hospital, Port-Harcourt.</p> <p><strong>Methodology:</strong> Venous blood was collected from study participants. Thick and thin blood films were made, stained with 10% dilution of Giemsa and blood films examined using microscope.</p> <p><strong>Results:</strong> Of the 200 participants, 151 (75.5%) were males and 49 (24.5%) females. Blood specimens analysis revealed that 57 (28.5%) participants were malaria parasites positive. Among them, 36(63.2%) were males and 21(36.8%) were females. The highest malaria prevalence, 11(42.3%) was observed in Nkporlu community and the least 15(21.1%) in Port-Harcourt Township. Malaria prevalence was highest among the age group of 46-55 years 6(66.7%) and least among age 26-35 years 14(16.7%) which was statistically significant (P&lt;0.05; P=0.000). Artisans and participants with primary education recorded highest prevalence of 6(100%) and 1(100.0%) respectively, while traders and those that attained secondary education had 9(18.8%) and 29(25.4%) respectively. There was a significant difference in malaria prevalence in relation to occupation (P&lt;0.05, <em>P</em> = 0.001) but not with educational status (P &gt; 0.05; P = 0.574). Malaria parasite intensities were mild 31(54.4%), moderate 24(42.1%) and heavy 2(3.5%), although not significant (P&gt;0.05).</p> <p><strong>Conclusion:</strong> Malaria screening should be made compulsory for all prospective blood donors throughout the country, despite the challenges posed by testing capacity constraints.</p> 2020-10-21T00:00:00+00:00 ##submission.copyrightStatement## Glutamate-Rich Protein Gene Sequences of a Rodent Malaria Parasite, Plasmodium berghei NK65, and Human Plasmodium falciparum Strains are Homologous 2020-11-17T03:25:28+00:00 Ishaya Yohanna Longdet Richard Joseph Kutshik Ngozi Yvonne Chibundu Bitrus Yakubu Dinci Tyem Davou <p><strong>Aim</strong>: To explore the molecular characteristics of glutamine rich protein (GLURP) gene in <em>Plasmodium berghei</em> NK65 and compare its genetic relatedness with those of Plasmodium falciparum. The GLURP is a key surface antigen and its gene a genetic marker for genotyping in malaria epidemiology.</p> <p><strong>Study Design:</strong> The design chose for this study was an experimental research.</p> <p><strong>Place and Duration:</strong> The study was undertaken in the Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos between October 2018 and June 2019.</p> <p><strong>Methods:</strong> Total Deoxyribonucleic acid was extracted from the whole blood of <em>Plasmodium berghei</em> NK65 infected mice using ZR Quick-gDNA™ Miniprep Kit (ZYMO RESEARCH). PCR was done using Gene Ampp9700. The amplicon was analyzed in 2% agarose gel, documented with ChemiGenuis® Gel Documentation System (Synegene) and sequenced at Inqaba Biotec Industries, South Africa. Finch TV® (GeoPiza) was used to access the GLURP nucleotide sequence and analysed using National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLASTn) and CLUSTAL O analyses tools.</p> <p><strong>Result:</strong> The polymerase chain reaction product, about 1kb, gave 863bp partial length GLURP gene of <em>Plasmodium berghei</em> NK65 strain. The bioinformatics analyses gave Blast Hits: <em>Plasmodium falciparum </em>GLURP gene (AF191065.1) with 98.23% identity; (AF247634.1) with 94.93% identity; and (XM_001347592.1) with 94.66% identity. This shows high similarity between <em>Plasmodium berghei</em> NK65 GLURP gene sequence and that of the human malaria parasite Plasmodium falciparum.</p> <p><strong>Conclusion:</strong> The presence of GLURP gene was reported for the first time in <em>Plasmodium berghei</em> NK65 which is homologous to strains of the deadliest human malaria parasite, Plasmodium falciparum.</p> 2020-11-17T00:00:00+00:00 ##submission.copyrightStatement## Prevalence and Intensity of Urinary Schistosomiasis among the Primary School Children of Some Selected Villages of Shinkafi Local Government Area, Zamfara State, Nigeria 2020-11-20T06:31:29+00:00 A. Y. Bala Salau, Shafa’atu H. M. Bandiya Mahmuda, Aliyu J. Suleiman <p><strong>Aim: </strong>The study was conducted to determine the prevalence and intensity of Urinary Schistosomiasis among Primary School children in selected villages of Shinkafi Local Government Area of Zamfara State, Nigeria.</p> <p><strong>Study Design:</strong> The study was designed to target a total of 400 primary school pupils from four (4) selected villages of the ten (10) districts of Shinkafi Local Government.</p> <p><strong>Place and Duration of Study:</strong> Badarawa, kware, Jengeru, and Kurya primary schools of Shinkafi local government area of Zamfara State, from August 2017 to March 2018.</p> <p><strong>Methodology:</strong> Four hundred (400) urine samples were collected from Primary Schools of Badarawa, Kware, Kurya and Jangeru villages using clean sample bottles covered with screw cup, the samples were examined visually and microscopically after filtration. All results obtained were statistically analyzed using analysis of variance (ANOVA) and binary logistic regression analysis.</p> <p><strong>Results:</strong> One hundred and seventy three (173) pupils were positive for <em>Schistosoma haematobium </em>given a prevalence of 43.3% with a mean egg intensity of 11.45 egg/10 ml of urine. A significantly higher infection rate of 76.0% with a higher intensity of 16.81 egg/10 ml of urine was recorded among pupils with blood-brown urine colour. Pupils from Badarawa primary schools accounted for the highest prevalence (73.0%) with also higher intensity of (26.94 egg/10 ml of urine) with significant association for the prevalence and significant difference for the mean egg intensity. Prevalence and intensity were observed to be not significant based on gender, age groups and parents occupations in the study areas.</p> <p><strong>Conclusion:</strong> Prevalence and intensity were significantly high based on urine colour and primary schools. Mass chemotherapy and awareness among the pupils and people in the study areas should be considered for future prevention measures.</p> 2020-11-20T00:00:00+00:00 ##submission.copyrightStatement##